Someone help me.. ps) I need cDNA library longer than 1.4kb and I can't buy commercail cDNA library because of being out of money.So cDNA library screening is not possible.
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#1
anonymous
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Posted 26 November 2001 - 10:00 PM
I got a partial cDNA sequence by cDNA library screening and 5'race... Because I can not get any more cDNA sequence by 5'race, I am gonna do genomic DNA library sequence.. but the problem is the way to find exon regions from genomic DNA fragment...
#2
anakinsdream30
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Posted 28 February 2002 - 06:06 PM
What kind of library? bacterial..cosmid....YAC? and the exons ur looking for...do u know the sequences...or is there sequence published? I was thinking that maybe u could end label a primer and screen it or make pCR primers specific for the exons in question and go that route...email me at anakinsdream30@aol.com...we can figure it out
Lonnie
