recommendation needed for a good p53 antibody
Posted 06 February 2009 - 09:58 AM
Posted 22 February 2009 - 12:35 PM
Can you tell me exactly which antibodies you've used? I ask because I work in a laboratory dedicated to p53 research so we've used many many anti-p53 antibodies over the years, for many different applications. So, to be any help I need to know 1) which antibodies you've tried 2) the exact protocols you've tried. The more details you can give, the better. It may also help if you could tell me who you got your antibodies from.
It's tricky because we're concerned with human p53, but there's a lot of experience in our lab with using mouse cell lines (not tissue) but I think if you can give some details about antibodies/protocols/etc we might be able to help.
Posted 22 February 2009 - 01:15 PM