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What is the biggest insert for a viral vector?


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#16 wincel

wincel

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Posted 24 September 2010 - 02:29 PM

Make sure you get the cDNA from the same tissue you plan to express it in under similar conditions. mRNA can be spliced very differently and this can be tissue specific and cell activation can cause the same effect. So your cDNA might look very different and have actually different properties than the one that is original for the tissue ...




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