I was trying to seed HUVEC in a 24-well plates for immunofluorescence (IF). The lab technician told me to put a piece of glass slide in the wells for possible high manification observation under microscope. And, as usually, we need to coat the substrate with gelatin for HUVEC. Then, in this case, I coated the glass slides. However, most of the cells just adhered the edge of the wells or the glass slides,; is seems like they don't like the slides. As HUVEC is monolayer cells, when there are too much cell at the edge, they started to dye while there is no cells on the centre of the slides. As I need to stain the protein in adherent junction of cells, this situation is apparently not good.
I tried many times and failed.
Does anybody here have good suggestion or experience here?
thanks in advance
Edited by haiyan, 06 February 2009 - 03:55 AM.