Posted 05 February 2009 - 02:20 PM
i am looking for media suggestions of people who have experience with hepatocytes. I am planning to buy the cells from Lonza and they are recommending some HCM media which is not common and very expensive. And they are very secretive about the media formulation (they jsut told me that it has no FBS). I have seen people using DMEM w/ FBS and w/o FBS in the literature. Would anybody have a suggestions about the media issue (FBS or no FBS, DMEM or HCM) or even hepatocyte culture in general...Thanks!
Posted 06 February 2009 - 07:03 AM
Edited by yobou, 06 February 2009 - 07:04 AM.
Posted 10 September 2009 - 12:01 PM
Posted 13 September 2009 - 04:27 PM
Hi, I work with rat and murine hepatocytes. We have been culturing them successfully in Waymouth'sMB 752/1 medium purchased from Gibco BRL. We add 5%BGS plus pen/strep.
i work with HepG2 cells and they grow easy in DMEM high glucose. Supplementation is easy: 1% L-glutamine, 10 %FBS, 1% antibiotics/antimycotic mix (avoid to using amphotericin B , recommend Itraconazole).
Renew the media in 2 days...... they will grow fine!
Posted 13 September 2009 - 04:33 PM
Don't use antibiotics in your culture unless you can help it - they mask all sorts of hidden contaminations which do affect your cells.