what is mock cell line?
Posted 04 February 2009 - 10:48 AM
Posted 04 February 2009 - 02:40 PM
guys, what is mock cell line? is it derived from human tissue or animal tissue? why is it being used so much for virus cultures? I can't find this info on ATCC's website or internet. believe me I searched everywhere.
a mock setting is a control setting where you do exactly the same as in your experimental set-up except you leave out the specific stimulus you want to provide. Maybe it's more comprehensible with an example: Let's say you want to study the effects of p53 overexpression in a cell line. You can do this by transfecting a plasmid with the p53 cDNA into the cell line. However, you have to make sure the effects you see are due to the p53 and not due to the plasmid backbone or the transfection procedure. So the mock cell line will be the same cell line as you use to introduce the p53 plasmid into, except in the mock setting you transfect the cells with an empty plasmid.
Hope I made it a little more clear ...
Posted 04 February 2009 - 02:52 PM
in the mock cell line you would add same volume of solvent to control for any effect of the solvent
Posted 15 April 2012 - 08:46 AM
Posted 08 June 2012 - 03:30 AM
That's what I understood, but maybe I am wrong...