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Old threads on "siRNA, microRNA and RNAi" are achived here
Started by bioforum, Feb 03 2009 11:27 PM
1 reply to this topic
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Posted 21 April 2010 - 04:23 PM
I am quite confused about the differences between the mechanisms by which (i) antisense oligonucleotides, (ii) long dsRNA, and (iii) short 21-23 dsRNA act on RNAs to affect gene expression in human cells.
I heard that long piece of dsRNA (in both worms or plants) activate the RNAi pathway, in where the long dsRNA gets cleaved into siRNA (20-25nt) and ultimately cause SPECIFIC mRNA degradation via RISC. Does this mean I should talk about the exogenous siRNA here? But long dsRNA cause broad/global effect!! I am confused...
In addition, both siRNA and miRNA are in definition a size of 19-25nt, where both have larger sized precursors. I do need clarification on these definitions. In addtion, isn't miRNA as a definition a endogenous single-stranded RNA?
I heard that long piece of dsRNA (in both worms or plants) activate the RNAi pathway, in where the long dsRNA gets cleaved into siRNA (20-25nt) and ultimately cause SPECIFIC mRNA degradation via RISC. Does this mean I should talk about the exogenous siRNA here? But long dsRNA cause broad/global effect!! I am confused...
In addition, both siRNA and miRNA are in definition a size of 19-25nt, where both have larger sized precursors. I do need clarification on these definitions. In addtion, isn't miRNA as a definition a endogenous single-stranded RNA?
