Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Functional Studies for Histone modifications


  • Please log in to reply
2 replies to this topic

#1 jiro_killua

jiro_killua

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 55 posts
0
Neutral

Posted 03 February 2009 - 06:43 PM

If I want to prove a causal relationship between histone H3K4me3 and a biological process, maybe the shraightforward reasoning would be to alter H3K4me3, and see if there's a resulting phenotype.

For example, if I'm doing DNA methylation, I can use something like 5-Aza

But is there a simple inhibitor for H3K4me3?

Or what better approach should I take (if possible)?

Please advice, thank!!

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
67
Excellent

Posted 03 February 2009 - 08:54 PM

Can you use RNAi to knock down the enzmye that is responsible for adding or removing H3K4me3. I know that recently several histone demethylases have been identified mostly by Yi Zhang's group.

#3 jiro_killua

jiro_killua

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 55 posts
0
Neutral

Posted 04 February 2009 - 12:10 PM

Can you use RNAi to knock down the enzmye that is responsible for adding or removing H3K4me3. I know that recently several histone demethylases have been identified mostly by Yi Zhang's group.


Thanks,

I guess a conceptual question is that if a gene is highly H3K4 trimethylated, if I use siRNA of histone methylase (I looked and found Wdr5, Rbbp5 and Ash2l) to knockdown the methylase, will H3K4me3 decrease?

I mean, is decrease of methylase enough?
Or whatever is methylated will stay there?
Is overexpression of demethylase (such as Jarid1c) necessary?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.