Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

knockout mouse with increase cell size


  • Please log in to reply
3 replies to this topic

#1 jiro_killua

jiro_killua

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 55 posts
0
Neutral

Posted 03 February 2009 - 06:34 PM

I was looking in Pubmed and MGI for knockout mouse models

I want to find a gene that when knocked out, will stop (or reduce) cell proliferation, but the cells in the mouse will continue to enlarge, such that the volume of an individual cell in the KO will be bigger than the wildtype

E.g. in drosophila, I've seen knockdown of Cdk1 will generate this phenotype, but I couldn't find a KO with this phenotype

Does anyone know of one?

If so, please let me know the paper that describe it.

Thanks a lot

#2 cellcounter

cellcounter

    Control Panel

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 145 posts
3
Neutral

Posted 05 February 2009 - 08:31 AM

I was looking in Pubmed and MGI for knockout mouse models

I want to find a gene that when knocked out, will stop (or reduce) cell proliferation, but the cells in the mouse will continue to enlarge, such that the volume of an individual cell in the KO will be bigger than the wildtype

E.g. in drosophila, I've seen knockdown of Cdk1 will generate this phenotype, but I couldn't find a KO with this phenotype

Does anyone know of one?

If so, please let me know the paper that describe it.

Thanks a lot

You should look for SKP2 knockout, the cells are double the size.

knockingout p27 in these mice reverts the size to normal.

Basically, all genes of this cell-cycle pathway have cell size relevance, so study SKP2, p27, CKS1 etc.

#3 jiro_killua

jiro_killua

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 55 posts
0
Neutral

Posted 05 February 2009 - 08:59 AM

I was looking in Pubmed and MGI for knockout mouse models

I want to find a gene that when knocked out, will stop (or reduce) cell proliferation, but the cells in the mouse will continue to enlarge, such that the volume of an individual cell in the KO will be bigger than the wildtype

E.g. in drosophila, I've seen knockdown of Cdk1 will generate this phenotype, but I couldn't find a KO with this phenotype

Does anyone know of one?

If so, please let me know the paper that describe it.

Thanks a lot

You should look for SKP2 knockout, the cells are double the size.

knockingout p27 in these mice reverts the size to normal.

Basically, all genes of this cell-cycle pathway have cell size relevance, so study SKP2, p27, CKS1 etc.



Great, thanks a lot

Skp2 KO are smaller in overall size too

The most interesting and ideal model would be a mouse with normal size overall, the individual cell size are doubled, and the total number of cells were halved.

Is that possible? :>

#4 cellcounter

cellcounter

    Control Panel

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 145 posts
3
Neutral

Posted 05 February 2009 - 09:02 AM

I was looking in Pubmed and MGI for knockout mouse models

I want to find a gene that when knocked out, will stop (or reduce) cell proliferation, but the cells in the mouse will continue to enlarge, such that the volume of an individual cell in the KO will be bigger than the wildtype

E.g. in drosophila, I've seen knockdown of Cdk1 will generate this phenotype, but I couldn't find a KO with this phenotype

Does anyone know of one?

If so, please let me know the paper that describe it.

Thanks a lot

You should look for SKP2 knockout, the cells are double the size.

knockingout p27 in these mice reverts the size to normal.

Basically, all genes of this cell-cycle pathway have cell size relevance, so study SKP2, p27, CKS1 etc.



Great, thanks a lot

Skp2 KO are smaller in overall size too

The most interesting and ideal model would be a mouse with normal size overall, the individual cell size are doubled, and the total number of cells were halved.

Is that possible? :>

I don't think that would be possible with mouse. It would not survive beyond organogenesis. Too complex a system. However, I may be wrong. With SKP2 too, it is mainly hepatocytes that show the phenotype if I remmber it correctly.

For your ideal conditions, it may be possible with drosophila larvae or C.elegans, or perhaps even xenopus or zebrafish embryos, but I would not expect such to happen in an adult mouse. I stand corrected if somebody knows this field better.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.