I have run some 2-D gels and have silver stained them. Now, I want to know which spot is my target protein; just wondering if I can western blot one of those 2-D gels. I am trying to stay away from running another 2-D.
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Is immunoblotting possible after the gel has been silver stained
Started by Opal, Feb 03 2009 03:27 PM
2 replies to this topic
#2
mdfenko
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Posted 04 February 2009 - 01:31 PM
not likely to work. i would just run a duplicate for blotting.
Edited by mdfenko, 04 February 2009 - 01:31 PM.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
Nrelo
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Posted 19 February 2009 - 01:49 AM
Opal, on Feb 3 2009, 03:27 PM, said:
I have run some 2-D gels and have silver stained them. Now, I want to know which spot is my target protein; just wondering if I can western blot one of those 2-D gels. I am trying to stay away from running another 2-D.
Probably no as the protein was being fixed
