Is immunoblotting possible after the gel has been silver stained
Posted 03 February 2009 - 03:27 PM
Posted 04 February 2009 - 01:31 PM
Edited by mdfenko, 04 February 2009 - 01:31 PM.
genius does what it must
i do what i get paid to do
Posted 19 February 2009 - 01:49 AM
I have run some 2-D gels and have silver stained them. Now, I want to know which spot is my target protein; just wondering if I can western blot one of those 2-D gels. I am trying to stay away from running another 2-D.
Probably no as the protein was being fixed