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Is immunoblotting possible after the gel has been silver stained


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#1 Opal

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Posted 03 February 2009 - 03:27 PM

I have run some 2-D gels and have silver stained them. Now, I want to know which spot is my target protein; just wondering if I can western blot one of those 2-D gels. I am trying to stay away from running another 2-D.

#2 mdfenko

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Posted 04 February 2009 - 01:31 PM

not likely to work. i would just run a duplicate for blotting.

Edited by mdfenko, 04 February 2009 - 01:31 PM.

talent does what it can
genius does what it must
i do what i get paid to do

#3 Nrelo

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Posted 19 February 2009 - 01:49 AM

I have run some 2-D gels and have silver stained them. Now, I want to know which spot is my target protein; just wondering if I can western blot one of those 2-D gels. I am trying to stay away from running another 2-D.


Probably no as the protein was being fixed




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