

Reuse DNA spin column
#1
Posted 03 February 2009 - 02:08 PM
#2
Posted 03 February 2009 - 06:08 PM
750 mM NaCl;
50 mM MOPS,
15% isopropanol (v/v);
0.15% Triton® X-100 (v/v)
pH 7.0;
#3
Posted 03 February 2009 - 08:51 PM
#4
Posted 05 February 2009 - 05:51 AM
Reuse DNA spin column
reuse_DNA_spin_column.pdf 440.14KB 4171 downloads
Thanks Minnie mouse to post my paper (i am one of the author of this paper)
#5
Posted 05 February 2009 - 01:19 PM
Reuse DNA spin column
reuse_DNA_spin_column.pdf 440.14KB 4171 downloads
Thanks Minnie mouse to post my paper (i am one of the author of this paper)
Thank you for publishing this paper. You have saved our money

If you have further tips on reusing the DNA spin column, please post them here?

We would love to know

#6
Posted 05 February 2009 - 02:37 PM
#7
Posted 07 February 2009 - 12:53 PM
http://openwetware.o...prep/Qiagen_kit
You might want to look at the Qiagen patent, which is linked off of that page as well.
#8
Posted 07 February 2009 - 02:55 PM
Welcome back phage434.
#9
Posted 21 March 2009 - 06:23 AM
Analytical Biochemistry
Volume 385, Issue 1, 1 February 2009, Pages 182-183
” Complete decontamination and regeneration of DNA purification silica columns”
#10
Posted 23 March 2009 - 01:56 PM
#11
Posted 26 March 2009 - 11:36 AM
#12
Posted 30 March 2009 - 11:56 AM
It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.
When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?
Also same thing about equilibrate. What does that mean exactly.
#13
Posted 13 April 2009 - 03:24 AM
#14
Posted 13 April 2009 - 02:55 PM
I am confused about the method exactly.
It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.
When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?
Also same thing about equilibrate. What does that mean exactly.
fill the column and centrifuge to get rid of the water
#15
Posted 14 April 2009 - 11:05 PM