22 replies to this topic

[Minnie Mouse]

Reuse DNA spin column

 reuse_DNA_spin_column.pdf   440.14KB   1642 downloads

[perneseblue]

QBT buffer

750 mM NaCl;
50 mM MOPS,
15% isopropanol (v/v);
0.15% Triton® X-100 (v/v)
pH 7.0;

[pcrman]

It is a nice find. I'm gonna give it a try.

[Apoptosiss]

Reuse DNA spin column

 reuse_DNA_spin_column.pdf   440.14KB   1642 downloads

Thanks Minnie mouse to post my paper (i am one of the author of this paper)

[Minnie Mouse]

Reuse DNA spin column

 reuse_DNA_spin_column.pdf   440.14KB   1642 downloads

Thanks Minnie mouse to post my paper (i am one of the author of this paper)

Thank you for publishing this paper. You have saved our money

If you have further tips on reusing the DNA spin column, please post them here?

We would love to know

[pcrman]

Has anyone tried reusing RNA columns such as Qiagen's RNeasy column?

[phage434]

The reconstitution papers referenced here fail to describe the N3 buffer used in the Qiagen minipreps. The guanidine hydrochloride used in buffers N3 and PB are not described, although these are important to binding DNA to the column. See this page for the composition of these buffers:

http://openwetware.o...prep/Qiagen_kit

You might want to look at the Qiagen patent, which is linked off of that page as well.

[Minnie Mouse]

Thank you for the link, phage434.

Welcome back phage434.

[labtek]

Other article for reuse DNA silica column:

Analytical Biochemistry
Volume 385, Issue 1, 1 February 2009, Pages 182-183
” Complete decontamination and regeneration of DNA purification silica columns”

[Minnie Mouse]

thank you, labtek

[torontostudent]

Any different recommendations for the Sigma GenElute columns. They come with a special column prep solution which I am not sure if it's the same as the Qiagen solutions.

[torontostudent]

I am confused about the method exactly.

It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.

When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?

Also same thing about equilibrate. What does that mean exactly.

[Fylhtq]

does anybody know about buffers from Macherei-Nagel NucleoSpin Blood L, NucleoSpin Plant L columns?

[Minnie Mouse]

I am confused about the method exactly.

It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.

When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?

Also same thing about equilibrate. What does that mean exactly.

fill the column and centrifuge to get rid of the water

[Nathan M]

I mentioned this to a TA of mine, and he said that 1.0 M NaOH would probably be just as good. I dunno, I have some OMEGA HiBind DNA mini-columns, I was wondering if it would be possible to use a different buffer to capture RNA instead?