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Posted 15 April 2002 - 07:55 AM
In DNA pool sequencing to caculate the allele frequence of a SNP, How many samples should I use and How can I make the DNA quality be equal?
Posted 07 June 2002 - 10:02 AM
Make sure all your DNA is equimolar by using a spectrophotomer then add the same ul amount of each for your pool. There is no fixed number that should be in your pool - less (ie 5) increases the odds of finding rare alleles while more allows you to scrren greater numbers of samples with the chances of missing a rarer allele here and there. See Wang et al Science 1998 280:1077-1082, Germer et al Genome Res 10:258-266, and Brouillette in Mam Gen Dec 2000.