Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Recommend a giant ladder please


  • Please log in to reply
4 replies to this topic

#1 Julio-Claudian

Julio-Claudian

    i'm unmethylated

  • Active Members
  • PipPipPipPipPip
  • 78 posts
6
Neutral

Posted 03 February 2009 - 06:40 AM

Dear all,

The largest marker I have in the lab is the Lambda HindIII. Can you guys recommend (name and manufacturer) any useful ladders for genomic DNA work and anything to do with the range in excess of 30kb?

Thanks!!
:) :) :P :P

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,742 posts
127
Excellent

Posted 03 February 2009 - 08:12 AM

how about this one from invitrogen:

high mw dna ladder
talent does what it can
genius does what it must
i do what i get paid to do

#3 hobglobin

hobglobin

    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,522 posts
95
Excellent

Posted 03 February 2009 - 09:16 AM

how about this one from invitrogen:

high mw dna ladder



Or NEB'S
λ DNA-Mono Cut Mix

Sometimes those companies offer trial offers for free. And they suffice a surprisingly long time.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#4 Julio-Claudian

Julio-Claudian

    i'm unmethylated

  • Active Members
  • PipPipPipPipPip
  • 78 posts
6
Neutral

Posted 04 February 2009 - 06:31 PM

Thank you mdfenko and hobglobin! Going for it!

#5 perneseblue

perneseblue

    Unlimited ligation works!

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 578 posts
17
Good

Posted 04 February 2009 - 07:23 PM

Or how about NEB pfg ladder. 1Mb to 48kb.
May your PCR products be long, your protocols short and your boss on holiday




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.