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Recommend a giant ladder please


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4 replies to this topic

#1 Julio-Claudian

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    i'm unmethylated

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Posted 03 February 2009 - 06:40 AM

Dear all,

The largest marker I have in the lab is the Lambda HindIII. Can you guys recommend (name and manufacturer) any useful ladders for genomic DNA work and anything to do with the range in excess of 30kb?

Thanks!!
:) :) :P :P

#2 mdfenko

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Posted 03 February 2009 - 08:12 AM

how about this one from invitrogen:

high mw dna ladder
talent does what it can
genius does what it must
i do what i get paid to do

#3 hobglobin

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Posted 03 February 2009 - 09:16 AM

View Postmdfenko, on Feb 3 2009, 06:12 PM, said:

how about this one from invitrogen:

high mw dna ladder


Or NEB'S
λ DNA-Mono Cut Mix

Sometimes those companies offer trial offers for free. And they suffice a surprisingly long time.
  
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#4 Julio-Claudian

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Posted 04 February 2009 - 06:31 PM

Thank you mdfenko and hobglobin! Going for it!

#5 perneseblue

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Posted 04 February 2009 - 07:23 PM

Or how about NEB pfg ladder.  1Mb to 48kb.
May your PCR products be long, your protocols short and your boss on holiday




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