6 replies to this topic

[than4]

I know this is probably a really basic question, but can someone please explain the difference between a neuroblast and a neurosphere?
Thanks

[TanyHark]

than4, on Feb 2 2009, 07:37 AM, said:

I know this is probably a really basic question, but can someone please explain the difference between a neuroblast and a neurosphere?
Thanks

Neuroblast I think is a stem cell.

[kfunk106]

In development, you have a cluster of cells that could differentiate into neuroblasts, which then turn differentiate into neurons. This cluster of cells is the neurosphere I think. Out of this group of cells only one actually differentiates into a neuroblast though, and this is established through the Delta-Notch signalling pathway. So the one that actually becomes the neuroblast will differentiate into a neuron and the rest will become other neural cells, like glia.

[ichatfilipina]

kfunk106, on Feb 7 2009, 03:28 AM, said:

In development, you have a cluster of cells that could differentiate into neuroblasts, which then turn differentiate into neurons. This cluster of cells is the neurosphere I think. Out of this group of cells only one actually differentiates into a neuroblast though, and this is established through the Delta-Notch signalling pathway. So the one that actually becomes the neuroblast will differentiate into a neuron and the rest will become other neural cells, like glia.

Neuroblast for? you said the cluster of celss is the neurosphere, then what will be the neuroblast?

[Pyaria]

neuroblast: stem cell dedicated to becoming a neuron

neurosphere: What you get when you grow neural stem cells in vitro

[gradstem]

My lab routinely cultures neurospheres. Neurospheres are when you dissociate embryonic brains and culture the cells non-adherently. The result is progenitor populations (typically either glial or neuronal) and a small percentage of neural stem cells that grow in spheres. The thought is that these progenitors proliferate in these spheres and the rate of proliferation is a result of the number of neural stem cells (although I do not this is not entirely accurate). I agree with the definitions of neuroblast.

[marktaylor]

than4, on Feb 2 2009, 06:37 AM, said:

I know this is probably a really basic question, but can someone please explain the difference between a neuroblast and a neurosphere?
Thanks

neuroblast is a dividing cell that will develop into neurons or glia..whereas as a neurosphere is asystem designed for neural stem cell