stabel Transfection of Jurkat cells
Posted 02 February 2009 - 03:01 AM
I tried a stable Transfektion of Jurkat cells via elektroporation. Unfortunately non of the cells died, not even in the mock transfected control.
No I tried a titration of the G418 but even at 5 mg/ml non of the Jurkats died, they grow and replicate very nicely....
So is it possible, that I use a Jurkat clone, that has already a G418 resistence? Something like in the 293T cells?
I can't find anything liek that in the www, perhabs you can help me.
Posted 04 February 2009 - 03:25 AM
Or it may be that the 293 is already selected using G418.
Posted 04 February 2009 - 05:04 AM