17 replies to this topic

[Bita]

What freezing medium is better for ADSCs (Adipose derived stem cells)?Should freezing medium be contained antibiotic or not?
helps are appreciated in advance

[Brahmavidhya Arun]

I have a basic question. whenever we prepare cell seed, we inoculate FTM for checking the sterility of cell seed.

Within 24 h. FTM changes to yellow with a thin line of pink ring at the top............i don't think it is contamination........... I inoculated FTM with serum alone, freezing medium and DMSO separately....but FTM resembles control.(no color change)......
please clarify my doubts..........


Thanks
Brahmavidhya K.V.

[bob1]

Brahmavidhya Arun, on 14 March 2012 - 12:33 AM, said:

Within 24 h. FTM changes to yellow with a thin line of pink ring at the top............i don't think it is contamination........... I inoculated FTM with serum alone, freezing medium and DMSO separately....but FTM resembles control.(no color change)......
please clarify my doubts..........

Can you please describe your procedure for this, that will help us to know what you have done and the likely causes of any problems.