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Western Blotting- A beginner Guide


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#1 Minnie Mouse

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Posted 01 February 2009 - 02:18 PM

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#2 Nitin k.Wadhwa

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Posted 10 September 2010 - 03:48 AM

I probed my protein (CD82 of 31Kda ) but got band at 60Kda.. that too a single band. what could be the reason for it please help......

#3 mdfenko

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Posted 10 September 2010 - 07:36 AM

it could be a dimer or, more likely, sds-page gives a decent estimate of molecular size for most proteins but some do not.
talent does what it can
genius does what it must
i do what i get paid to do

#4 GNANA

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Posted 17 September 2010 - 02:49 PM

may be non specific, jus to confirm ip down ur protein and run simultaneoulsy along with the total lysate and probe it...may be you will get a solution...

good luck,
gnana..,
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....




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