Edited by Sayeh, 30 January 2009 - 01:20 PM.
How to sterilize cover slips (glasses)?
Posted 30 January 2009 - 01:19 PM
Posted 30 January 2009 - 03:43 PM
Posted 30 January 2009 - 06:04 PM
The UV is silly- it's pretty good at blocking.
Very good point. We got contamination by just UV irradiation.
Posted 31 January 2009 - 12:28 AM
Posted 31 January 2009 - 06:30 AM
Posted 31 January 2009 - 12:48 PM
Posted 31 January 2009 - 12:52 PM
pure (100%?) alcohol is less effective and more expensive than 70%.
Yup. there were several good threads on this. 70% was recommended due to several reasons.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 01 February 2009 - 07:35 AM
Posted 01 February 2009 - 04:56 PM
Posted 01 February 2009 - 09:39 PM
I drop them in pure alcohol, leave them for 5 min, remove them and wash them with PBS 3x to remove alcohol....I also used to UV them for 1 hour, but not anymore cause I see no contamination.
Are you Iranian?
Posted 02 February 2009 - 09:38 AM
Posted 02 February 2009 - 11:35 AM
In a pinch, I guess spraying with ETOH would work out.
Posted 02 February 2009 - 01:58 PM
Posted 04 February 2009 - 01:22 AM
leave it in the autoclave for an extra few minutes after the cycle, the heat in the autoclave will dry it up.
Posted 04 February 2009 - 11:44 AM
The option for dry materials, has a drying cycle at the end of its sterilization cycle. that eliminates the "drying step".