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How to sterilize cover slips (glasses)?


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#1 Sayeh

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Posted 30 January 2009 - 01:19 PM

Hi, I am wondering how you sterilize cover glasses? In my old lab we used to wash them really good and then rinse them with 70% ethanol and place them under UV for 30 min. Do you know a better alternate?

Edited by Sayeh, 30 January 2009 - 01:20 PM.


#2 GeorgeWolff

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Posted 30 January 2009 - 03:43 PM

For mycology slides cultures - wash them in surfactant to remove grease, 70% ethanol and flame. The UV is silly- it's pretty good at blocking.

#3 pcrman

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Posted 30 January 2009 - 06:04 PM

The UV is silly- it's pretty good at blocking.


Very good point. We got contamination by just UV irradiation.

#4 Curtis

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Posted 31 January 2009 - 12:28 AM

I drop them in pure alcohol, leave them for 5 min, remove them and wash them with PBS 3x to remove alcohol....I also used to UV them for 1 hour, but not anymore cause I see no contamination.

movafagh bashid

#5 rkay447

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Posted 31 January 2009 - 06:30 AM

If it's just plain, uncoated coverglass we put them in glass petri dishes and autoclave them.

#6 GeorgeWolff

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Posted 31 January 2009 - 12:48 PM

pure (100%?) alcohol is less effective and more expensive than 70%.

#7 hobglobin

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Posted 31 January 2009 - 12:52 PM

pure (100%?) alcohol is less effective and more expensive than 70%.


Yup. there were several good threads on this. 70% was recommended due to several reasons.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

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#8 scolix

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Posted 01 February 2009 - 07:35 AM

One can dip the coverslip in 100% ethanol and burn them one by one. Takes a lot of time. Or autoclave them.

#9 LostintheLab

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Posted 01 February 2009 - 04:56 PM

We autoclave a pack of coverslip usually, but if there's no time spraying with 70% ETOH and drying (in the sterile hood obviously) worked very well too.

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#10 Sayeh

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Posted 01 February 2009 - 09:39 PM

I drop them in pure alcohol, leave them for 5 min, remove them and wash them with PBS 3x to remove alcohol....I also used to UV them for 1 hour, but not anymore cause I see no contamination.

movafagh bashid



Are you Iranian?

#11 gebirgsziege

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Posted 02 February 2009 - 09:38 AM

I never tried, but 180C for 30 - 60 min???? I am not sure if they survive, but this usually is very effective....
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#12 labrat612

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Posted 02 February 2009 - 11:35 AM

My lab would usually spray with 70% ETOH then autoclave.

In a pinch, I guess spraying with ETOH would work out.


~Labrat

#13 Sangeet

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Posted 02 February 2009 - 01:58 PM

Autoclaving I think is not a good idea for coverglasses cause then you have to dry up them all.
180o for 120 min is the scientific way i know and apply.

#14 scolix

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Posted 04 February 2009 - 01:22 AM

If one autoclaves coverslip, you do have to dry it, but its no problem.

leave it in the autoclave for an extra few minutes after the cycle, the heat in the autoclave will dry it up.

#15 labrat612

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Posted 04 February 2009 - 11:44 AM

we have an autoclave with two cycles: one that's just for liquids and other for dry things, such as coverslips, forceps, etc.

The option for dry materials, has a drying cycle at the end of its sterilization cycle. that eliminates the "drying step".




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