3 replies to this topic

[BadKarma]

Dear all,

I need some help! What software use to analyze MALDI-TOF data?

Thanks in advance

[T C]

Hey

MALDI-data?

Are you trying to sequence yr protein, if yes then u got to submit it to mascot. We have a GPS explorer software which does it for us. Alternatively take the peak list and submit here:

http://prospector.ucsf.edu/cgi-bin/msform....m=mstagstandard

However, this is just MS based search and u would get better coverage and confidence if you search using both MS and MS/MS data in GPS explorer or protein pilot.

Hope it helps

TC

[BadKarma]

T C, on Jan 31 2009, 05:30 AM, said:

Hey

MALDI-data?

Are you trying to sequence yr protein, if yes then u got to submit it to mascot. We have a GPS explorer software which does it for us. Alternatively take the peak list and submit here:

http://prospector.ucsf.edu/cgi-bin/msform....m=mstagstandard

However, this is just MS based search and u would get better coverage and confidence if you search using both MS and MS/MS data in GPS explorer or protein pilot.

Hope it helps

TC

Yes only did MALDI-Tof analysis. we want find a phosphorylated residue and decided use MALDI as a first approach... Bad idea?

Thanks for reply

[T C]

Well

Phosphorylations are tricky....people use dedicated instruments like Qtrap (4000 trap) for analysis of post translational modifications. MALDI can do it, but is difficult. Atleast you would see a difference in masses.

I know people who use MALDI to just see if their in vitro phosphorylation has worked and then use Qtrap. These people are also optimizing conditions. Also, they use IMAC columns to enrinch.

Hope it helps.

TC