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Phosphoproteins


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#1 BadKarma

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Posted 30 January 2009 - 04:20 AM

Dear all,

Does anyone have already use Phos-tag technology to analyse phophorylated proteins? I'm looking for kits to separate this proteins from a crude extract and I'm wondering if any of you are familiarized with this

Thanks in advance
"Ever tried. Ever failed. No matter. Try again. Fail again. Fail better." Samuel Becket

#2 neuron

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Posted 30 January 2009 - 07:30 AM

I have not used such kits but I've heard about phospho-enrichment IMAC columns.I thought they are used to separate phosphopeptides. Anyways when i was looking for some of these kits, i found one with pierce, if it could help.-

http://www.piercenet...05-E62ECAACC2A7




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