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Caspase substrates for flow cytometry


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#1 thegradstudent

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Posted 29 January 2009 - 02:48 PM

Hi there!!!

I have a question. What's in the "flow cytometry dilution buffer" that needs to be used along with PhiPhiLux Substrates? Could I use standard "flow cytometry staining buffer"??

Thanks
TheGradStudent

#2 jiro_killua

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Posted 09 February 2009 - 07:35 PM

Hi there!!!

I have a question. What's in the "flow cytometry dilution buffer" that needs to be used along with PhiPhiLux Substrates? Could I use standard "flow cytometry staining buffer"??

Thanks


That's for washing the cells, though I don't quite understand what you meant by flow cy staining buffer
You might probably substitute it with PBS, and it used to work for me, though I haven't done it for ages

It comes with the kit and why not just use it?




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