41 replies to this topic

[mirmir]

tea-test, on Jan 29 2009, 09:46 AM, said:

Hello,

I have gathered some important documents with many useful information regarding real time PCR.

Great!! thanks for all that

[nanan]

Thank you very much and you are really a iron man, I think.

[chrystea]

thank you very much for the articles. i find them very useful. it helped a lot! thanks again  

[Laurence]

Wow,so good.....

[sud373]

tea-test, on Jan 29 2009, 11:20 PM, said:

tea-test, on Jan 29 2009, 07:46 PM, said:

Hello,

I have gathered some important documents with many useful information regarding real time PCR.

here are the next ones

thanx for gvng such informative papers..

[Lulupz]

Hello!
Anyone have the next paper?
Bonefeld BE, Elfving B, Wegener G: Reference genes for normalization: a study of rat brain tissue.
Synapse 2008, 62:302-309.
Please!!
Thanks

[tea-test]

check your pm.

[sanjiun]

Guys,

Like MIAME for microarray paper, MIQE is a useful guideline for those who want to do qPCR.

Quote

The aim of MIQE, coordinated by a group of research-active scientists and coordinated under the umbrella of MIBBI (Minimum Information for Biological and Biomedical Investigations) is to provide authors, reviewers and editors specifications for the minimum information that must be reported for a qPCR experiment in order to ensure its relevance, accuracy, correct interpretation and repeatability. A checklist, which should be submitted along with the paper, is available for authors in preparing a manuscript employing qPCR.

Here is the website: http://www.rdml.org/miqe.php

Attached Files

•  miqe_bustin_et_al_clin_chem_2009.pdf   117.9K   171 downloads
•  MIQE_checklist.xls   35.5K   144 downloads

Edited by sanjiun, 04 December 2009 - 01:48 AM.

[Dcienmeder]

very useful and thx a lot

[flammaefata]

The following article I found especially interesting concerning use of reference genes for normalization.  Please note that I have already highlighted this article - sorry if that is bothersome!

Thanks for all the other ones you posted!

 EDIT_2009___Normalization_of_qRT_PCR_data___The_necessity_of_adopting_a_systematic__experimental_conditions_specific__validation_of_references.pdf   781.54K   239 downloads

[phosphate girl]

This is great tea-test thank-you so much! I have just started molecular biology and qPCR so this is amazing!

[phosphate girl]

Thanks also Sanjiun and flammaefata - such a great help!

[liudm0103]

It should be very usefull, thanks a lot!

[baccilus]

Thank you very much for these compiled protocols. I would like to read something about HRM curve analysis using SYBR green on an Applied Biosystem 7500 RT-PCR. Actually that is the PCR in my Lab and I have to do HRM with it.
Any help regarding this would be greatly appreciated.
Thank you in advance.

[vampire001]

thank you for these papers！