41 replies to this topic

[tea-test]

Hello,

I have gathered some important documents with many useful information regarding real time PCR.

Attached Files

•  Application_note_Understanding_Ct.pdf   2.03MB   1933 downloads
•  Accurate_normalization_of_real_time_quantitative_RT_PCR_data_by_geometric_averaging_of_multiple_internal_control_genes.pdf   141.4K   1151 downloads
•  Analysis_of_Relative_Gene_Expression_Data_Using_Real_Time_Quantitative_PCR_and_the_2_deltadeltaCT_Method_.pdf   713.66K   1253 downloads

[tea-test]

tea-test, on Jan 29 2009, 07:46 PM, said:

Hello,

I have gathered some important documents with many useful information regarding real time PCR.

here are the next ones

Attached Files

•  APPLICATION_NOTE_TaqMan__Gene_Expression_Assays.pdf   212.62K   610 downloads
•  Bio_Rad_Application_Guide_Real_Time_PCR_Bulletin_5279B.pdf   2.16MB   858 downloads
•  Data_Analysis_on_the_ABI_PRISM__7700_Sequence_Detection_System_Setting_Baselines_and_Thresholds.pdf   354.95K   552 downloads

[tea-test]

here's the next bunch

Attached Files

•  nolan_hands_bustin_2006_Quantification_of_mRNA_using_real_time_RT_PCR.pdf   971.99K   590 downloads
•  pfaffl_a_new_mathematical_model_for_relative_quantification_in_real_time_pcr.pdf   119.46K   461 downloads
•  qBase_relative_quantification_framework_and_software_for_management_and_automated_analysis_of_real_time_quantitative_PCR_data.pdf   983.75K   435 downloads
•  Statistical_significance_of_quantitative_PCR.pdf   433.55K   693 downloads

[tea-test]

and even more

Attached Files

•  The_implications_of_using_an_inappropriate_reference_gene_for_real_time_reverse_transcription_PCR_data_normalization.pdf   132.97K   498 downloads
•  ABI_User_bulletin__2_Rev_B.pdf   483.22K   487 downloads
•  ABI_user_bulletin__5.pdf   226.57K   427 downloads
•  Processing_of_gene_expression_data_generated_by_quantitative_real_time_RT_PCR.pdf   106.43K   618 downloads

[tea-test]

I think it would be a good idea to make this a sticky thread and everyone who knows interesting literature can post it here.

Thank You!

[bioforum]

Thank you for compiling a very useful list of papers related to real-time PCR. Your topic has been pinned.

[Jeffrey]

very thx for ur help!

[appleyun]

Wow! Thanks for all that!

[smilealie]

hey thanx for all the basic information.

[tea-test]

Here is another interesting brochure about real time PCR from Invitrogen:

Attached Files

•  INVITROGEN_REAL_TIME_PCR_From_Theory_To_practice.pdf   1.34MB   795 downloads

[lamb]

From Finnzymes' website you can order a free copy of "Principles of qPCR". It includes basics of chemistries, instruments, assays and main applications. There's a link to the ordering form at the lower right of their web page.

http://www.finnzymes...ents_index.html

[ryan]

thanks, these articles are fantastic for someone who has never done any qPCR

[grassgirl]

I'm looking for literature on gene expression analysis using absolute quantification to detect RNAi suppression in plants.

Thank you for any suggestions.

[ulswau]

Thank  you for your paper, They are just what I want to learn.

[jiajia1987]

Hi everyone,

Is it just me or what? whenever i click on the papers, all I get is a blank page!!