Important literature for real time PCR users
Posted 11 November 2010 - 02:07 PM
I am wondering whether any of you can help me with some of my questions. I have a total RNA consists of bacterial and human ribosomal RNA as well as mRNA. I would like to quantify the 16S, 18S, rpoD and ACTB genes in this sample before and after my attempt to remove ribosomal RNA with the Ambion MicrobeEnrich/MicrobeExpress kit. I am expecting 16S, 18S and ACTB to go down after the removal and rpoD should stay the same (or slight reduced due to RNA lost throughout the process). I have all the qPCR numbers but I do not how to make sense of them. How can I calculate the rpoD enrichment-fold from the copies number that I have from qPCR?
Posted 26 January 2011 - 08:23 AM
Posted 07 April 2011 - 05:55 AM
It uses the already present results from thousands of microarray datasets to identfy the most stable expressed genes in, for instance, a certain tissue or a certain type of cancer.
Posted 15 May 2011 - 05:29 PM
Thank you so much for your kindness in sharing scientific knowledges among us. As I am very beginner in real time PCR, I found your attachment extremely helpful and informative. I odore you as u have resolved many problems concerned with some other fellows. Thank you so much.
Posted 29 November 2011 - 04:56 AM
Do you guys know this paper published in 2009 titled as 'A simple and loss-free method to remove TRIzol contaminations from minute RNA samples'?
At least I observed that the RNA purity increased a lot after purification following this simple and cheap protocol.
Link is below;
Here is the article:
Krebs et al cleanup of TRIzol from RNA.pdf 217.88KB 1012 downloads
Edited by Liinda, 29 November 2011 - 04:59 AM.
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