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Important literature for real time PCR users


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41 replies to this topic

#16 mirmir

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Posted 23 September 2009 - 03:00 AM

Hello,

I have gathered some important documents with many useful information regarding real time PCR.




Great!! thanks for all that

#17 nanan

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Posted 24 September 2009 - 07:11 AM

Thank you very much and you are really a iron man, I think.

#18 chrystea

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Posted 02 October 2009 - 12:43 AM

thank you very much for the articles. i find them very useful. it helped a lot! thanks again ;)

#19 Laurence

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Posted 11 October 2009 - 12:49 AM

Wow,so good..... :huh:

#20 sud373

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Posted 30 October 2009 - 02:39 AM

Hello,

I have gathered some important documents with many useful information regarding real time PCR.


here are the next ones


thanx for gvng such informative papers..
suddu

#21 Lulupz

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Posted 30 October 2009 - 09:40 PM

Hello!
Anyone have the next paper?
Bonefeld BE, Elfving B, Wegener G: Reference genes for normalization: a study of rat brain tissue.
Synapse 2008, 62:302-309.

Please!!
Thanks

#22 tea-test

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Posted 02 November 2009 - 06:03 AM

check your pm.
tea-test: The artist formerly known as Ned Land

#23 sanjiun

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Posted 04 December 2009 - 01:47 AM

Guys,

Like MIAME for microarray paper, MIQE is a useful guideline for those who want to do qPCR.

The aim of MIQE, coordinated by a group of research-active scientists and coordinated under the umbrella of MIBBI (Minimum Information for Biological and Biomedical Investigations) is to provide authors, reviewers and editors specifications for the minimum information that must be reported for a qPCR experiment in order to ensure its relevance, accuracy, correct interpretation and repeatability. A checklist, which should be submitted along with the paper, is available for authors in preparing a manuscript employing qPCR.


Here is the website: http://www.rdml.org/miqe.php

Attached Files


Edited by sanjiun, 04 December 2009 - 01:48 AM.


#24 Dcienmeder

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Posted 19 December 2009 - 07:19 PM

very useful and thx a lot

#25 flammaefata

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Posted 24 February 2010 - 06:30 AM

The following article I found especially interesting concerning use of reference genes for normalization. Please note that I have already highlighted this article - sorry if that is bothersome!

Thanks for all the other ones you posted!

Attached File  EDIT_2009___Normalization_of_qRT_PCR_data___The_necessity_of_adopting_a_systematic__experimental_conditions_specific__validation_of_references.pdf   781.54KB   362 downloads

#26 phosphate girl

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Posted 08 March 2010 - 04:41 AM

This is great tea-test thank-you so much! I have just started molecular biology and qPCR so this is amazing!

#27 phosphate girl

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Posted 08 March 2010 - 04:44 AM

Thanks also Sanjiun and flammaefata - such a great help!

#28 liudm0103

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Posted 30 March 2010 - 07:37 PM

It should be very usefull, thanks a lot!

#29 baccilus

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Posted 24 August 2010 - 10:16 PM

Thank you very much for these compiled protocols. I would like to read something about HRM curve analysis using SYBR green on an Applied Biosystem 7500 RT-PCR. Actually that is the PCR in my Lab and I have to do HRM with it.
Any help regarding this would be greatly appreciated.
Thank you in advance.

#30 vampire001

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Posted 09 November 2010 - 12:15 AM

thank you for these papers!




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