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CHO cells


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4 replies to this topic

#1 srd1

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Posted 29 January 2009 - 07:04 AM

Hello

I'm hoping someone would be able to advise me on where to buy CHO cells. I am interested in working with CHO cells dieficient in galactosyltransferase/xylosyltransferase or heparan sulfate polymerisation. I know these cells are available from ATCC, however I would like to see if I can get them cheaper elsewhere. I can't find these cells on the ECACC website and I was wondering if there is another likely source I could look into?

Thanks for any suggestions

#2 labrat612

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Posted 29 January 2009 - 07:50 AM

Have you tried Invitrogen?
I've gotten some of their suspension-adapted CHO cells.

Or perhaps Sigma-Aldrich- they also use the EACC. And if you're in Europe you are able to purchase those cell lines through them.

Hope that helps.

Labrat

#3 rkay447

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Posted 29 January 2009 - 06:41 PM

Hello

I'm hoping someone would be able to advise me on where to buy CHO cells. I am interested in working with CHO cells dieficient in galactosyltransferase/xylosyltransferase or heparan sulfate polymerisation. I know these cells are available from ATCC, however I would like to see if I can get them cheaper elsewhere. I can't find these cells on the ECACC website and I was wondering if there is another likely source I could look into?

Thanks for any suggestions

Not sure where you work or if there are other labs around you but I've gotten a number of cell lines for free by just asking around. Our university also has a tissue culture/baculovirus core facility which cells over 100 verified/micoplasm free cell lines at $11 for a frozen vial. I find that most people are actually unaware that this core facility offers cell lines so be sure to ask around where you work!

#4 labrat612

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Posted 09 February 2009 - 08:09 AM

Thanks for any suggestions[/quote]
Not sure where you work or if there are other labs around you but I've gotten a number of cell lines for free by just asking around. Our university also has a tissue culture/baculovirus core facility which cells over 100 verified/micoplasm free cell lines at $11 for a frozen vial. I find that most people are actually unaware that this core facility offers cell lines so be sure to ask around where you work!
[/quote]

Really?? I am one of those poor ignorant souls who never knew that! Do you know if they would sell to biotech companies? If so, please tell me how to get in touch with this facility.

~Labrat

#5 rkay447

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Posted 09 February 2009 - 08:15 AM

Sorry but this facility is for the university only. But, it really wouldn't be that hard to check out your local university and see if you can find a lab working with CHO cells there. Another possible method is to find someone who has published a stable CHO cell line that would be of interest to you. You can then request the cell line from the group. It's a bit odd to ask for a common cell line such as the CHO cells alone but once someone makes a stable cell line and publishes, that's fair game for requests.




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