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a question about RT-PCR


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#1 mcyst

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Posted 30 May 2002 - 06:20 PM

We extracted total RNA from mouse liver and spleen.The OD260/OD280 of the RNA is 2.0. The electrophoresis result is three bands.But when we use the RNA to conduct PCR without first reverse transcription , we still can obtain a very clear band that is similar to the result of RT-PCR. What's the possible reason? Thank you!

#2 scogne

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Posted 30 May 2002 - 10:04 PM

Hi,

maybe you have important genomic DNA contamination. Do you performed DNAse I (RNAse free) treatment?





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