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PCR quantification


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#1 lukefreedman

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Posted 28 January 2009 - 06:46 AM

Hello all wise peoples. Im conducting end point PCRs using a number of different primers pairs which im comparing to a baseline reading primer constant between conditions. Im told that using image J and some of its associated plugins i can quantify my pcr bands and and produce ratios relative to the baseline reading which can then be compared to each other. I had a search on the web but didnt realy manage to find any real help. Can anyone help, either by explaining the process itself or pointin me in the direction of someone/something that can? Any assistance would be realy appreciated, quantifying my results would add much weight to the project im doing.


Thanks.

Luke

#2 pcrman

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Posted 03 February 2009 - 09:01 PM

For quantitating PCR bands, imageJ is not a very uesful tool. If you can find a old program called Gel Lab, it will help a lot.

You should go for real qPCR.




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