2 replies to this topic

[dna_nerd]

anybody want to walk me through (or point in the right direction) how best to set up a qpcr run to both validate the integrity of my INPUT samples and ensure my primers are functional?

thanx

[yarince]

dna_nerd, on Jan 28 2009, 01:38 AM, said:

anybody want to walk me through (or point in the right direction) how best to set up a qpcr run to both validate the integrity of my INPUT samples and ensure my primers are functional?

thanx

Try this article...

http://www.plantmeth...6-4811-3-11.pdf

[dna_nerd]

yarince, on Jan 29 2009, 02:05 PM, said:

dna_nerd, on Jan 28 2009, 01:38 AM, said:

anybody want to walk me through (or point in the right direction) how best to set up a qpcr run to both validate the integrity of my INPUT samples and ensure my primers are functional?

thanx

Try this article...

http://www.plantmeth...6-4811-3-11.pdf

thanks yarnice, that was a good read

i have tried two different methods to calculate the fold change of my ChIP sample versus the INPUT sample (after normalizing for the no antibody sample of course) and achieved the same results

i understand what that is telling me, but don't quite get the idea of % INPUT, when I calculate it I get very small numbers (good?) but can't currently place any meaning towards it

any input? (sorry, lame joke)