Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?
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#2
scolix
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Posted 01 February 2009 - 07:12 AM
BioSaint, on Jan 27 2009, 05:51 PM, said:
Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?
That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?
#3
BioSaint
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Posted 02 February 2009 - 01:32 AM
That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?
*** Starting with localization.. FITC is ok.. But any other stuff?
#4
scolix
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Posted 04 February 2009 - 03:29 AM
Have you thought about using GFP fused to A-beta? They may not work but they could also work.
#5
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Posted 25 March 2009 - 01:48 AM
Yeah, thought about it mate, but it may harm the aggregation pattern..
