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Amyloid beta labelling


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4 replies to this topic

#1 BioSaint

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Posted 27 January 2009 - 07:51 AM

Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?

#2 scolix

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Posted 01 February 2009 - 07:12 AM

Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?


That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?

#3 BioSaint

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Posted 02 February 2009 - 01:32 AM


That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?


*** Starting with localization.. FITC is ok.. But any other stuff?

#4 scolix

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Posted 04 February 2009 - 03:29 AM

Have you thought about using GFP fused to A-beta? They may not work but they could also work.

#5 BioSaint

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Posted 25 March 2009 - 01:48 AM

Yeah, thought about it mate, but it may harm the aggregation pattern..




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