I was just wondering if anyone can help me. I have been amplifying a gene in three fragments and so far two out of the three have been successful. The problem is the C-Terminal end which just refuses to work. Apparently the primers are fine (as I came into the project after the primers were designed) but on my gel I have noticed a bright band which is tiny in size. Do you think this could be primer dimer or non-specific amplification? Im trying to see if my supervisor will let me design my own primers but they seem convinced that that would do no good. Its not just me, a college of mine cant get it to work either...
Any suggestions would be greatly appreciated!
P.s the two fragments that have worked were 2 and 3 kb but the one that wont work is 4kb... I was also wondering if the size could be a problem?
Edited by Kami23, 27 January 2009 - 07:49 AM.