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pGEX


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2 replies to this topic

#1 saraarasus

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Posted 27 January 2009 - 12:50 AM

Hi,
has any one tried to express pGEX in the eucayotic cells or any comments?

#2 Beta Campos

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Posted 31 January 2009 - 05:31 AM

I have many probems with pGEX4T1 vector too....
Now I think that BAD choice.... Have a others best vectors...
I need 2600 bp cloning insert with BamHI + SmaI digest and nothing at last!
My cells are eucaryotic too...

Best,

Beta.
Roberta Campos

#3 rkay447

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Posted 31 January 2009 - 06:38 AM

The pGEX vectors are not designed or meant for protein expression in eukaryotic cells. They do not have a promoter for eukaryotes. They are designed for expression in prokaryotes and therefore have the correct sequences for prokaryotic transcription. These vectors are meant for expressing your protein in bacteria and will not work in eukaryotic cells.




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