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Housekeeping gene selection for copy number validation using qRT-PCr


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#1 Thoracic

Thoracic

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Posted 26 January 2009 - 06:09 PM

HI,

I am trying to validate the copy number variations of two targets selected via microarray. I want to use qRT-PCR for quantification using genomic DNA. Does anybody have any advice as to how to select a good housekeeper for relative quantification? I know that the housekeeper should have normal copy number i.e is diploid but how do I select one? Does anybody have any suggestions as to what I sould use?

Some people have said that ideally you should select a housekeeper from teh same chromosome.

Any help would be great.




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