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What does 'limiting dilution PCR' mean?


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4 replies to this topic

#1 phytoqueen

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Posted 26 January 2009 - 02:04 PM

Probably what I'm doing is a limiting dilution PCR, but what does that mean??

#2 swanny

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Posted 26 January 2009 - 02:15 PM

Probably what I'm doing is a limiting dilution PCR, but what does that mean??

Can you tell us what you're doing?

I had always understood it to be a dilution series of the template DNA to determine when amplification effectively stops. Apart from anything else, it's good to use to see if you have an inhibitor in the template DNA.
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#3 phytoqueen

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Posted 28 January 2009 - 04:52 PM

Thank you. Then what I'm doing is not a limiting dilution PCR. I misunderstood..

#4 swanny

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Posted 28 January 2009 - 10:09 PM

OK, so what are you doing?
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#5 gebirgsziege

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Posted 28 January 2009 - 11:06 PM

Yes.....what are you doing, I would be interested in that as well.......
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)




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