1 reply to this topic

[anonymous]

Hello,

I am working with a set of primers which require RNase prior to PCR.  I have made several attempts however,in all cases I have also lost most if not all of the DNA.  Does anybody have any suggestions or knowwhat conc. the RNase should have of the final volume.

Thanks,

Mark

[anonymous]

This may be a stupid question but is your RNase DNase free? Are you using more or less than 10ng/ul?