RT-PCR - can you keep the PCR plate in the fridge?
Posted 26 January 2009 - 04:31 AM
So glad the forum is back up running! I was just wondering if anyone has any experience of leaving a PCR plate in the fridge prior to running the PCR? Would my plate be ok with the samples and primers in it for about 4 hours before I run it on the pcr machine? And then should I wait for it to return to room temp before running it? Any help would be much appreciated! Thanks!
Posted 26 January 2009 - 08:10 AM
you shouldn't need to prewarm the plate to room temperature, the cycler will take care of all temperature changes.
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