Hek 293 cell line with tetra/doxycycline induction
Posted 26 January 2009 - 02:15 AM
Our lab has bought Invitrogen Flp-In T-rex cells line 1 year ago and we now got our clones. The problem is that the promotor seems to be very strong and our protein aggregated so we want to reduce the expression level. The manuscript of them recommended to use 0.1-1µg/ml Tetracycline or similar conc. of Doxycycline to induced the cell line. Currently we are using 0.1µg/ml. I have asked their technics but they also have no clue and only recommended me to buy their FBS, which is Tetracycline free
Does anyone has experience with this cell line and do you know till which concentration of Tetracycline can we get half or even less expression level?
Thanks a lot if anyone would like to help!
Posted 01 February 2009 - 07:40 AM
Posted 09 February 2009 - 02:26 PM