How to culture PC3 cell line
Posted 26 January 2009 - 01:30 AM
Thaks for your replies.
Posted 26 January 2009 - 03:47 AM
Posted 26 January 2009 - 04:06 AM
PC-3 cells are pretty easy to culture. We use RPMI-1640 and change medium every 3-4 days.
Is it normal, that colour of the medium changes so much?
And one more question about sub-culturing: how do you subc-ulture these cells? From 70-80% confluent cells 1:5 or rather 1:10?
Posted 27 January 2009 - 05:27 AM
I've done 1:5 splits before.