3 replies to this topic

[jtd]

Hi, I work at Warsaw University in Molecular Biology Department. I just started working with PC3 cell line  and I have some questions to somebody who works with this line. What kind of medium do you use? In cell line data shet I found Coon's mosified Ham's F12 medium, but in many publication I foudn RPMI 1640.  How often You change the medium? And How often do you passage the cells?
Thaks for your replies.
Joanna

[pcrman]

PC-3 cells are pretty easy to culture. We use RPMI-1640 and change medium every 3-4 days.

[jtd]

pcrman, on Jan 26 2009, 01:47 PM, said:

PC-3 cells are pretty easy to culture. We use RPMI-1640 and change medium every 3-4 days.

Is it normal, that colour of the medium changes so much?

And one more question about sub-culturing: how do you subc-ulture these cells? From 70-80% confluent cells 1:5 or rather 1:10?

[claire9]

If the media is changing colour so much you should probably consider performing a media change about half way between passages.
I've done 1:5 splits before.