Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to culture PC3 cell line


  • Please log in to reply
3 replies to this topic

#1 jtd

jtd

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 26 January 2009 - 01:30 AM

Hi, I work at Warsaw University in Molecular Biology Department. I just started working with PC3 cell line and I have some questions to somebody who works with this line. What kind of medium do you use? In cell line data shet I found Coon's mosified Ham's F12 medium, but in many publication I foudn RPMI 1640. How often You change the medium? And How often do you passage the cells?
Thaks for your replies.
Joanna

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,157 posts
65
Excellent

Posted 26 January 2009 - 03:47 AM

PC-3 cells are pretty easy to culture. We use RPMI-1640 and change medium every 3-4 days.

#3 jtd

jtd

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 26 January 2009 - 04:06 AM

PC-3 cells are pretty easy to culture. We use RPMI-1640 and change medium every 3-4 days.



Is it normal, that colour of the medium changes so much?

And one more question about sub-culturing: how do you subc-ulture these cells? From 70-80% confluent cells 1:5 or rather 1:10?

#4 claire9

claire9

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 27 January 2009 - 05:27 AM

If the media is changing colour so much you should probably consider performing a media change about half way between passages.
I've done 1:5 splits before.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.