Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

siRNA transfection into 3T3-L1


  • Please log in to reply
No replies to this topic

#1 Gin

Gin

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 31 March 2005 - 07:05 AM

Hi everyone,
I have done siRNA transfection into 3T3-L1 cell lines (pre-adipocytes) several times with siRNA made from our Lab and RNAiFect Transfection Reagent from Qiacen. However, I found that the cells couldn't differentiated well after transfected (over 90% non-treated cells got fully differentiation compared with 60-70% in the case of transfected cell).

Is there anyone has done the same my experiment now. Could you help me how to solve the problem?

Many thanks.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.