I have done siRNA transfection into 3T3-L1 cell lines (pre-adipocytes) several times with siRNA made from our Lab and RNAiFect Transfection Reagent from Qiacen. However, I found that the cells couldn't differentiated well after transfected (over 90% non-treated cells got fully differentiation compared with 60-70% in the case of transfected cell).
Is there anyone has done the same my experiment now. Could you help me how to solve the problem?
siRNA transfection into 3T3-L1
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