3 replies to this topic

[searcher]

For the buffering system should i also add CO2 into the medium or only NaHCO3 into the medium?



Thanks for any input.

[fred_33]

hi
usually, medium contains all required. For CO2/HCO3 buffering, it only comes from the 5% of CO2 in the air of the cell storer.

[searcher]

fred_33, on Mar 31 2005, 06:22 AM, said:

hi
usually, medium contains all required. For CO2/HCO3 buffering, it only comes from the 5% of CO2 in the air of the cell storer.

<{POST_SNAPBACK}>

What if the medium i buy does not have NaHCO3? Should i have to add NaHCO3 into it? I don't need to add CO2 because it comes from the 5% CO2 gass right?

[knuf]

Your best bet is just to ask the supplier what is needed to be added. Generally, you just have to add FBS and some antibiotics unless you have a special cell line like primary neurons or are using an inducible system or something like that...but I'venever had to add any sort of buffering ingredient.