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CO3 / HCO3 content of the culture medium


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3 replies to this topic

#1 searcher

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Posted 31 March 2005 - 12:21 AM

For the buffering system should i also add CO2 into the medium or only NaHCO3 into the medium? :)



Thanks for any input.

#2 fred_33

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Posted 31 March 2005 - 05:22 AM

hi
usually, medium contains all required. For CO2/HCO3 buffering, it only comes from the 5% of CO2 in the air of the cell storer.

#3 searcher

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Posted 31 March 2005 - 10:36 AM

hi
usually, medium contains all required. For CO2/HCO3 buffering, it only comes from the 5% of CO2 in the air of the cell storer.

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What if the medium i buy does not have NaHCO3? Should i have to add NaHCO3 into it? I don't need to add CO2 because it comes from the 5% CO2 gass right? :(

#4 knuf

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Posted 10 February 2009 - 07:23 AM

Your best bet is just to ask the supplier what is needed to be added. Generally, you just have to add FBS and some antibiotics unless you have a special cell line like primary neurons or are using an inducible system or something like that...but I'venever had to add any sort of buffering ingredient.




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