I am sick of trial and error loading!

Posted 30 March 2005 - 06:18 PM
Posted 31 March 2005 - 06:01 PM
I am after a protein quantitation method which is ok to use when there is detergents in your solubilisation/lysis buffer. I would normally use the Biorad protein assay (Lowry assay), but I am getting massive errors (always increased estimates) of my protein concentrations. I have been using trizol to extract the protein so I assume it is due to a carry-over of phenol (when I don't use trizol, my lowry assay works well) - is there a quantitation assay which I can use if I have some phenol contamination?
I am sick of trial and error loading!
Posted 31 March 2005 - 06:21 PM
Posted 30 January 2009 - 07:50 AM
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