2 replies to this topic

[molcyt]

Hi,
when I work with DNA to detect copy number changes, what kind of reference should I use? Shall I use GAPDH or a different gene which shows normal in the whole genome?

Is there any difference to design primer for DNA and cDNA? I mean for cDNA, my primers were located in two different exons. How will I design primer for DNA? Thank you very much.

[yongyk]

Dear molcyt,

Are you working with gene expression?


Regards
yong

[molcyt]

Dear Yong,

No, I am working with copy number  change detection (amplification, deletion). For this reason I have to work with DNA.