DNA copy number-QPCR
Posted 30 March 2005 - 08:21 AM
when I work with DNA to detect copy number changes, what kind of reference should I use? Shall I use GAPDH or a different gene which shows normal in the whole genome?
Is there any difference to design primer for DNA and cDNA? I mean for cDNA, my primers were located in two different exons. How will I design primer for DNA? Thank you very much.
Posted 30 March 2005 - 12:02 PM
Are you working with gene expression?
Posted 30 March 2005 - 12:15 PM
No, I am working with copy number change detection (amplification, deletion). For this reason I have to work with DNA.