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DNA copy number-QPCR


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#1 molcyt

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Posted 30 March 2005 - 08:21 AM

Hi,
when I work with DNA to detect copy number changes, what kind of reference should I use? Shall I use GAPDH or a different gene which shows normal in the whole genome?

Is there any difference to design primer for DNA and cDNA? I mean for cDNA, my primers were located in two different exons. How will I design primer for DNA? Thank you very much.

#2 yongyk

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Posted 30 March 2005 - 12:02 PM

Dear molcyt,

Are you working with gene expression?


Regards
yong

#3 molcyt

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Posted 30 March 2005 - 12:15 PM

Dear Yong,

No, I am working with copy number change detection (amplification, deletion). For this reason I have to work with DNA.




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