All negative effects of His-Tag and c-myc epitope?
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Posted 30 March 2005 - 03:58 AM
I'm planning to use a vector with His-Tag and c-myc epitope at the C-terminal of my protein. Can you please tell me all kinds problems I could face, esp., how possible is it to effect my protein activity, protein folding, glycosylation in yeast expression system, antibody interactions ? waiting hopefully for all answers...