Hi, smoeone told me tha for Libate PCR product into some complex vector(for example pEGFP) is a good idea to make a by pass step into an easyest vector as pGEM or Bluescript...
Someone has just tryed this protocol? I'm not so confident about this tecnique and i'd like to know if it's a good idea.
thanks for every hepl!
Best regards and good work!!!
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Posted 30 March 2005 - 01:13 PM
The only reason I see for that is to make use of more unique restriction sites in the multi-cloning region of the above 'general cloning' vectors. Basically, its an aid in your cloning scheme so you don't end up doing complex digests and ligations.
Otherwise, if you have the needed sites, then I would go straight into your final vector.
Otherwise, if you have the needed sites, then I would go straight into your final vector.
