I've had problems with some site-directed mutagenesis (following Strategene's Quikchange II protocols) and tried using the Quikchange II XL kit that is basically the same outside the inclusion of "QuikSolution" reagent. I noticed a marked increase in mutant generation (i.e. from 0 colonies to over 100) with high efficiency (~100%). Does anyone know the contents of this magical reagent? Strategene is tight-lipped about the proprietary reagent and only offered that too much of it will reduce the fidelity of the polymerase. Is it Mg2+ based? Or something else . . . ?
0
Quiksolution
Started by wkim2000, Mar 29 2005 07:40 PM
No replies to this topic
