Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Quiksolution


  • Please log in to reply
No replies to this topic

#1 wkim2000

wkim2000

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 29 March 2005 - 07:40 PM

I've had problems with some site-directed mutagenesis (following Strategene's Quikchange II protocols) and tried using the Quikchange II XL kit that is basically the same outside the inclusion of "QuikSolution" reagent. I noticed a marked increase in mutant generation (i.e. from 0 colonies to over 100) with high efficiency (~100%). Does anyone know the contents of this magical reagent? Strategene is tight-lipped about the proprietary reagent and only offered that too much of it will reduce the fidelity of the polymerase. Is it Mg2+ based? Or something else . . . ?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.