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[erguo918]

we use oligo-capping method.
mRNA is ligased with a RNA oligo at the 5' end,first.
RNAoligo(5'－AGCAUCGAGUCGGCCUUGUUGGCCUACUGG－3')
RT is performed with the oligo-dt primer
oligo-dT adapter primer2(5'－GCGGCTGAAGACGGCCTATGTGGCC(T)20－3')
PCR is performed in the procedure below
94 5' 94 1' 58 1' 68 10' 68 10' 30cycles
PCR PRIMER IS 5'pirmer A (5'－AGCATCGAGTCGGCCTTGTTG－3')
3'primer B (5'－GCGCTGAAGACGGCCTATGT－3' )

then digest the vector and the cDNA with SFI1
and then ligasion
but the clone is wrong.