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RNA and DNA ladders

Started by vetticus3, Mar 29 2005 04:56 PM

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#1 vetticus3

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Posted 29 March 2005 - 04:56 PM

Hi,
I'm working in RNA at the moment, and we don't have an RNA ladder... but we've been told that a DNA ladder works just as well, especially if its just to confirm the fidelity of the RNA. So, i ran the an formaldehyde and agarose gel, and the DNA ladder didn't show up.
i was wondering, WHY? is this because, i might have mixed up the loading buffer with the ladder (possible? yes).... or that DNA and agarose/formaldehyde gels don't get along?

Thanks,
vetticus

edit: apparently using loading dye instead of a ladder will produce no results. DNA ladder works just fine. Sorry for the hassle.

Edited by vetticus3, 29 March 2005 - 09:46 PM.

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