The best temperature would be 4 degree Celcius. Once you make a column like this you don't want it to be too long before you use it, within a week or two. You can always reuse them as well, as long as you get a good elution and are not stripping the protein off. As for what buffer, I would suggest either a tris or phosphate buffer with added Sodium Azide to inhibit bacterial growth.
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anonymous
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Posted 22 October 2001 - 09:00 PM
Hello, I want to store GST fusion protein fixed on glutathion sepharose beads to use it later for pull-down assay. I don't want to eluate it from beads for storage. So I want to know which kind of buffer I can use to store GST fusion protein fixed on glutathion sepharose beads and at which temperature?
