and method B formula is; Cell concentration per milliliter = Total cell count in 5 squares x 50,000 x dilution factor. Where did he get 2500 in A and 50,000 in B?

http://www.animal.uf...macytometer.htm
Thanks for any inputs.
Posted 29 March 2005 - 04:18 PM
Posted 29 March 2005 - 09:32 PM
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 30 March 2005 - 03:35 AM
hi justwonder,
the haemocytometer takes up a fixed volume.
There are squares of different sizes reflecting a fixed fraction of the volume.
Depending on which squares you use to count the cells a multiplication factor is required. Put simply, method B uses the smaller squares for cell counting hence the larger multiplication factor, while in method A the large squares are used.
Nick
Posted 30 March 2005 - 05:03 PM
What do you think about the methods? Usually we take the average of the counted squares, but these methods don't do it. Any ideas?
Thanks.
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
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